Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.
Identifieur interne : 00DD98 ( Main/Exploration ); précédent : 00DD97; suivant : 00DD99Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.
Auteurs : M V Reddy [Inde] ; K A Parkhe ; W F Piessens ; B C HarinathSource :
- Journal of clinical laboratory analysis [ 0887-8013 ] ; 1989.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Anticorps monoclonaux, Antigènes d'helminthe (immunologie), Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filarioses, Humains, Larve (immunologie), Technique de Western, Test ELISA, Wuchereria (immunologie), Wuchereria bancrofti (immunologie).
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Antigènes d'helminthe, Filariose lymphatique, Larve, Wuchereria, Wuchereria bancrofti.
- sang : Anticorps antihelminthe.
- Animaux, Anticorps monoclonaux, Filarioses, Humains, Technique de Western, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antibodies, Monoclonal, Antigens, Helminth (immunology), Blotting, Western, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Filariasis, Humans, Larva (immunology), Wuchereria (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , immunology : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Larva, Wuchereria, Wuchereria bancrofti.
- Animals, Antibodies, Monoclonal, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Filariasis, Humans.
Abstract
The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.
PubMed: 2681621
Affiliations:
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sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1989">1989</date><idno type="RBID">pubmed:2681621</idno><idno type="pmid">2681621</idno><idno type="wicri:Area/PubMed/Corpus">006061</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">006061</idno><idno type="wicri:Area/PubMed/Curation">006061</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">006061</idno><idno type="wicri:Area/PubMed/Checkpoint">006061</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">006061</idno><idno type="wicri:Area/Ncbi/Merge">007D90</idno><idno type="wicri:Area/Ncbi/Curation">007D90</idno><idno type="wicri:Area/Ncbi/Checkpoint">007D90</idno><idno type="wicri:doubleKey">0887-8013:1989:Reddy M:wb:e:monoclonal</idno><idno type="wicri:Area/Main/Merge">00EA15</idno><idno type="wicri:Area/Main/Curation">00DD98</idno><idno type="wicri:Area/Main/Exploration">00DD98</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.</title><author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra</wicri:regionArea><wicri:noRegion>Maharashtra</wicri:noRegion></affiliation></author><author><name sortKey="Parkhe, K A" sort="Parkhe, K A" uniqKey="Parkhe K" first="K A" last="Parkhe">K A Parkhe</name></author><author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name></author><author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></author></analytic><series><title level="j">Journal of clinical laboratory analysis</title><idno type="ISSN">0887-8013</idno><imprint><date when="1989" type="published">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antibodies, Monoclonal</term><term>Antigens, Helminth (immunology)</term><term>Blotting, Western</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Filariasis</term><term>Humans</term><term>Larva (immunology)</term><term>Wuchereria (immunology)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Anticorps monoclonaux</term><term>Antigènes d'helminthe (immunologie)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Filarioses</term><term>Humains</term><term>Larve (immunologie)</term><term>Technique de Western</term><term>Test ELISA</term><term>Wuchereria (immunologie)</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Larve</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Larva</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Filariasis</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Filarioses</term><term>Humains</term><term>Technique de Western</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name><name sortKey="Parkhe, K A" sort="Parkhe, K A" uniqKey="Parkhe K" first="K A" last="Parkhe">K A Parkhe</name><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name></noCountry><country name="Inde"><noRegion><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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